Intramural Projects - (Ongoing)

1. Project title: Antioxidant and anti-inflammatory activities of the Caesalpinia bonducella seed formulation.

Objective: To investigate the antioxidant activity and anti-inflammatory effect of Caesalpinia bonducella seed formulation in Wistar rats.

About the project: The study aims at evaluating the properties of Caesalpinia bonducella seed formulation by studying the effect on anti-oxidant enzymes and anti-inflammatory markers

Expected outcome: Model establishment, Ph.D. thesis, Publication

 2.  Project title: Establishment of the chicken egg as an alternate CDX model to study human cancer cell proliferation and metastasis.

Objective: To develop chorio allantonic membrane mediated tumour xenograft models in fertilized chicken eggs.

About the Project:  The study aim at developing the simple, cost-effective and maintenance-free human tumour xenograft model using fertilized chicken egg. The CAM mediated tumour xenograft model will form a base to test the anti-cancer agent for its efficacy against cancer cell survival, tumorigenicity and metastasis.

Expected outcome: Model establishment, Service

3. Project title: In vitro evaluation of a synthetic bone implant

Objective: To evaluate the bone implant preparations in vitro

About the Project:  The bone implant preparations were studied in the rat Mesenchymal progenitor cell-derived osteoblast in vitro. The cytotoxic effect of bone implant material was studied by MTT as per the Guidance Document on Good In Vitro Method Practices (GIVIMP).  Osteoblast differentiation was studied by alkaline phosphatase activity.

Expected outcome: Model establishment, Service

4.  Project title: Prospective analysis of Intrinsic factors promoting Benign Prostatic Hyperplasia – a pilot study

Objective: To investigate the prospective analysis of Intrinsic factors promoting Benign Prostatic Hyperplasia

About the Project:  The prospective analysis of Intrinsic factors promoting Benign Prostatic Hyperplasia – a pilot study is undertaken to generate evidence and a demographic impact. 

Expected outcome: Model establishment, Publication

5. Project title: Isolation of scardovia wiggisae in children with severe early childhood caries and caries-free children using real-time polymerase chain reaction.

Objective: To isolate scardovia wiggisae in children with severe early childhood caries and caries-free children using real-time polymerase chain reaction.

About the Project:  The isolation of scardovia wiggisae and comparing it between caries-free children and child with caries will help to understand the genomics and influence personalized medication for factors influence of caries in early childhood.

Expected outcome: PG Dissertation, Service, Publication

6. Project title: FABP based diagnostic kit for the early detection of MI.

Objective: To develop a FABP based diagnostic kit for the early detection of MI.

About the Project:  Development of simple self-detection kit based on salivary fatty acid binding protein (FABP) will help for early detection of myocardial infarction. Salivary biomarker protein(s) are being studied for early detection of myocardial infarction using bottom-up proteomics.

Expected outcome: Model establishment, Publication

7.  Project title: Salivary protein(s) biomarker for breast cancer: a non-invasive approach using proteomics.

Objective: To study the salivary protein(s) biomarker for breast cancer, a non-invasive approach using proteomics.

About the Project:  Protein profiling of breast cancer patients saliva will help to screen the candidate biomarker proteins for staging and early detection of breast cancer. The outcome of the project would enable us in the development of a simple and quick self-test kit for a benign and malignant tumour.

8. Project title: Establishment of  a lab animal reduction programme (Regional Animal Sample Archive-RASA)

Objective: To establish an innovative approach of banking of animal samples for lab animal reduction programme

About the Project:  The samples of the animal study conducted in the facility are being stored in appropriate condition for the use of the samples in the future by seekers of this region. The banking service is offered for the tissue samples of this region to abide by the 3R rule.

Expected outcome: Repository, Service

9. Project title: Sodium Valproic acid-induced Polycystic ovary syndrome (PCOS) in rat  -Pilot Study

Objective: To investigate the Sodium Valproic acid-induced Polycystic ovary syndrome (PCOS) in rat

About the Project:  The Polycystic ovary syndrome (PCOS) was induced in Wistar rats by Sodium Valproic acid. The PCOS induction is studied by hormone assay, histopathological examination and in vitro folliculogenesis.

Expected outcome: Model establishment, Ph.D. thesis, Publication

10.  Project title: Isolation and characterization of peptide pheromone from MRSA

Objective: To Isolate and characterize the peptide pheromone from MRSA

About the Project:  Identification of active pheromone compounds from MRSA will help to develop an attractant and/or repellent of such organism

Expected outcome: Model establishment, Product, Publication

11.  Project title: Evaluation of salivary changes and DNA damage in Buccal epithelial cells among individuals residing near fixed base trans-receiver station – an observational study.

Objective: To evaluate the salivary changes and DNA damage in Buccal epithelial cells among individuals residing near fixed base trans receiver station

About the Project:  The observational study on the evaluation of salivary changes and DNA damage in Buccal epithelial cells among individuals residing near fixed base trans receiver station will help to understand the environmental changes induced effects in the humans.

Expected outcome: Ph.D. thesis, Publication

12. Project title: Isolation and characterization of progenitor cells from rat bone marrow and PBMC

Objective: To Isolate and characterize progenitor cells from rat bone marrow and PBMC

About the Project:  The Mesenchymal stem cells were isolated from rat bone marrow and delineated into osteoblast. The peripheral blood mononuclear cells were isolated and delineated into multinucleated macrophages for osteoclast formation. The delineated progenitor cells are further subjected to cytotoxic and efficacy studies.

Expected outcome: Model establishment, Service,  Publication

13.  Project title: Effect of change in light-dark cycle on beta cell function and FoxO localization in Fisher 344 animal model.

Objective: To investigate the effect of change in light-dark cycle on beta cell function and FoxO localization in Fisher 344 animal model.

About the Project:  The study is to understand the change in sleep pattern leading to type 2 diabetes with specific reference to the relationship between FOXO localization, CLOCK and pancreatic markers/ loss of β cell function in Fisher 344 animal model.

Expected outcome: Model establishment, PDF, Publication

14.  Project title: Morphological, biochemical and genetic characterization of Down syndrome

Objective: To study the morphological, biochemical and genetic characterization of Down syndrome

About the Project:  The morphological, biochemical and genetic characterization involved in Down syndrome will help to identify new unknown factor to develop a target therapy.

Expected outcome: Ph.D. thesis, Service, Publication

15. Project title: Studies on the effect of the traditional formulation of C. bounducella seeds on Polycystic ovarian syndrome in rat model

Objective: To evidence the effect of the traditional formulation of C. bounducella seeds on Polycystic ovarian syndrome in rat model.

About the Project:  The traditional formulation of C. bounducella seeds influence on Polycystic ovarian syndrome is being evaluated in rat model by hormone assay, histopathological examination and in vitro folliculogenesis.

Expected outcome: Ph.D. thesis, Publication

Intramural Projects - (Completed)

1. Project Title: Applications of Terminalia arjuna (Roxb.) Wight & Arn leaf extract in wound healing.

Objective: To evaluate the wound healing potential of Terminalia arjuna leaf extract in vivo.

About the Project: Wound healing potential of the methanolic extract of the leaf of Terminalia arjuna was evaluated in rat excision and incision wound model. The study was performed according to the FDA guidelines.

Outcome: model established, manuscript under preparation

Intramural

2. Project Title: Evaluation of anti-inflammatory and anti-arthritic properties of the extract of   the leaf of Terminalia arjuna (Roxb.) Wight & Arn in vivo.

Objective: To evaluate the anti-inflammatory and anti-arthritic properties of the extract of   the leaf of Terminalia arjuna (Roxb.) Wight & Arn in vivo.

About the Project: Objective: The evaluation of the anti-inflammatory and anti-arthritic properties of the extract of   the leaf of Terminalia arjuna (Roxb.) Wight & Arn will be a therapeutic solution with fewer side effects than conventional therapy. 

Outcome: model established, Ph.D thesis research work, manuscript under preparation

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3. Project Title: Garlic infused ghee (GIG) as a modulator of lipid metabolism

Objective: To develop the scientific evidence for the lipid modulator effect of GIG.

About the Project: The study was performed to evaluate the anti-hyperlipidemic potential of GIG using high-fat diet induced hyperlipidemic rat model.

Outcome: model established, validation research work, under preparation, extended study planned

4.  Project Title: In vitro cell viability assessment using acridine orange/ethidium bromide staining.

Objective: To evaluate H9C2 cell adhesion and viability on synthetic nanomaterial in vitro.

About the Project: The study evaluated the compatibility of the synthetic material for the adhesion and growth of H9C2 cells. Acridine orange and ethidium bromide staining were used to evaluate the compatibility of synthetic materials in invitro.

Outcome: model established, sevices

5.  Project title: In vitro hemocompatibility of synthetic materials.

Objective:  To evaluate the hemocompatibility of the synthetic nanomaterial in vitro.

About the Project: The study evaluated the compatibility of synthetic nanomaterial with human blood in vitro condition. The study also forms a base for testing materials for their hemocompatibility as per ASTM guidelines.

Outcome: model established, service, One research article published

6.  Project title: Does active focus of attention to music alleviate patient’s anxiety under regional anaesthesia: a randomized controlled study

Objective:  To measure perioperative anxiety levels by STAI (Shortened Tamil version)  and biochemical markers  salivary Amylase, cortisol and secretory immunoglobulin A in the patient preferred music experimental group before and after the music listening intervention

About the Project: The project is aimed towards the investigation of the biological and psychological impact of the surgical environment on the conscious adult patient undergoing elective surgery under regional anaesthesia.

Outcome: One manuscript under preparation, inter-departmental collaboration

7. Project title: To validate the in vitro effect of Terminalia arjuna in in vivo osteoporosis animal models

Objective:  To validate the in vitro effect of methanolc extract of bark of Terminalia arjuna in in vivo osteoporosis animal models

About the Project: The animals fed with high sucrose and high fat diet induced osteoporosis were treated with the methanolic extract of bark of Terminalia arjuna and also investigated the estrogenic effect of T. arjuna in C57BL/6J mice.

Outcome: model established, manuscript under preparation

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8. Project title: Biocompatibility testing of biomaterials

Objective: To test the osteocompatability of the synthetic biomaterials

About the project: The osteocompatability of the biomaterials were evaluated by estimating calcium by alizarin red staining. ASTM guidelines were followed.

Outcome: model established, service, One research article published

9.  Project title: In vivo mammalian micronucleus assay – validation study

Objective: To perform in vivo mammalian micronucleus assay in mouse model using cyclophosphamide and mitomycin-c

About the project: The study served as a base for testing items/compounds for genetic toxicity using micronucleus assay. The study enabled the protocol development and standardization of micro nucleus assay in mouse model as per OECD guideline 474.

Outcome: model established, validation study for service

10. Project title: In vitro bacterial reverse mutation assay – validation study

Objective: To study the in vitro bacterial reverse mutation assay – validation study

About the project: The study is validation study for the facility following OECD guidelines Ames Test, OECD 471 

Outcome: model established, validation study for service

11. Project Title: Acute oral toxicity study of TA leaf extracts in rats.

Objective: To determine the adverse effect of TA leaf extract in in vivo using rat model.

About the Project: The study forms the base for determining the concentration at which the extract of leaf of Terminalia arjuna becomes toxic to rats as per OECD guidelines 420.

Outcome: model established, validation study for service, manuscript under preparation

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12. Project Title: Objective: To determine the adverse effects of oral administration of acetaminophen in in vivo.

Objective: To determine the adverse effects of oral administration of acetaminophen in in vivo

About the project:The study formed the base for testing the item/material in in vivo for its acute toxicity. The study enabled the development and standardization of the protocol to perform acute oral toxicity as per OECD guidelines.

Outcome: model established, validation study for service

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13. Project Title: Acute oral toxicity study of the herbal formulation of C. bonducella in vivo.

Objective: To determine the acute toxicity of the herbal formulation of C. bonducella in vivo using rat model.

About the Project: The study forms the base for determining the concentration at which herbal formulation of C. bonducella becomes toxic to rats as per OECD guidelines 420.

Outcome: model established, Ph.D thesis, manuscript under preparation

14. Project Title: Evaluation of Anti-granuloma of the leaf of an extract of Terminalia arjuna (Roxb. Wight & Arnot in cotton-pellet induced granuloma in the rodent model.

Objective: To evaluate the anti-granuloma of the leaf of an extract of Terminalia arjuna (Roxb.) Wight & Arn not in cotton-pellet induced granuloma in the rodent model.

About the Project: The anti-inflammatory activity of Terminalia arjuna was evaluated using cotton pellets-induced granuloma in the rat model as per OECD Guideline 423.

Outcome: model established, Ph.D thesis, manuscript under preparation

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15. Project Title: Aspirin and Pylorus ligation induced Gastric Ulcer in Rats –validation study

Objective: To study the Aspirin and Pylorus ligation induced Gastric Ulcer in Rats –validation study

About the Project: The study was designed to evaluate aspirin and pylorus ligation-induced ulcer model in Wistar rats.  The Gastric protection was evaluated by measuring the ulcer index, total acidity of gastric juice and gastric wall mucus thickness. In the present study, gastric ulcers have induced by aspirin plus pylorus ligation in Wistar rats as per OECD Guidelines 425

Outcome: model established, validation study for services

16.  Project Title: Acetic acid induced writhing assay in rat

Objective: To evaluate the antinociceptive effect of acetic acid in the rat.

About the Project: The antinociceptive effect was tested by using the acetic acid-induced writhing model in the rat as per OECD guidelines No. 423.

Outcome: model established, validation study for services

17. Project Title: Evaluation of Marine extracts cytotoxicity in HCT-116

Objective: To evaluate the cytotoxicity of the marine compounds/extracts using MTT in HCT-116 (Human colorectal carcinoma) cell line

About the Project: The study forms the base for the development and standardization of the protocol to test and determine the IC50 of marine extracts/items in vitro as per Guidance Document on Good In Vitro Method Practices (GIVIMP)

Outcome: model established, validation study for services, manuscript under preparation

18. Project title: Evaluation of synthetic compound PLUR cytotoxicity in HCT-116

Objective: To evaluate the cytotoxicity of synthetic compound PLUR using MTT in HCT-116 (Human colorectal carcinoma) cell line.

About the Project: The study evaluated the cytotoxicity of the synthetic compound in vitro using MTT.  The study optimized the protocol and method to determine IC50 of the synthetic compound as per Guidance Document on Good In Vitro Method Practices (GIVIMP) OECD guidelines No. 286

Outcome:, service, collaborative research, manuscript under preparation

19. Project title: Anti-Inflammatory effect of the marine extract on Carrageenan induced paw-oedema in a rat model.

Objective: To experiment with the Anti-Inflammatory effect of the marine extract on Carrageenan induced paw-oedema in a rat model.

About the Project: The Anti-Inflammatory effect of the marine extract on Carrageenan induced paw-oedema in a rat model as per OECD guidelines 420

Outcome: model established, SBV collaboration, manuscript under preparation

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