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CAReTS, SBV

CENTER FOR ANIMAL RESEARCH TRAINING AND SERVICES​ (CAReTS)

A Center of

Sri Balaji Vidyapeeth (SBV), puducherry

Regional Small Animal Research Facility for Preclinical Studies and Services Established under the joint initiative of Department of Biotechnology, Government of India and Sri Balaji Vidyapeeth

SERVICES​

Types of Services provided so far with beneficiaries and institutions

  1. Small animal Histopathology Services – Department of Biotechnology, Pondicherry University
  2. Nanomaterial Hemocompatibility Services – Department of Physics, Pondicherry University
  3. SNP analysis PCR Services – ICMR Student Research Project, Mahatma Gandhi Medical College and Research Institute, Puducherry
  4. Viral load Real-Time PCR Services – NPDF Research Project, Rajiv Gandhi Institute of Veterinary Education and Research, Puducherry
  5. PCR Methodology, budgeting consultation services – PG dissertation, Indira Gandhi Institute of Dental Sciences, Puducherry
  6. Histopathology Services  – PhD Thesis, Bharathiar University, Coimbatore
  7. Zebra Fish histopathology Services – PhD Thesis, Annamalai University, Chidambaram
  8. Nanomaterial Cell Culture Services  – St. Joseph’s College of Arts and Science, Cuddalore
  9. Plant Histopathology Services – Department of Biotechnology, Pondicherry University, Puducherry
  10. Haematology Services  – Department of Biotechnology, Pondicherry University, Puducherry
  11. Immunohistochemistry Services  – PhD Thesis, Bharathiar University, Coimbatore
  12. Immunohistochemistry Services – JSS College of Pharmacy, Mysuru
  13. MTT assay Services – PhD Thesis, CIDRF, Puducherry
  14. Nucleic acid quantization Services – PhD Thesis, CIDRF, Puducherry
  15. Analytical Services – PG dissertation, Indira Gandhi Institute of Dental Sciences, Puducherry
  16. Sample Freeze Storage Services – Mahatma Gandhi Medical College and Research Institute, Puducherry
  17. RT PCR Services – PhD Thesis, CIDRF, Puducherry

Research / Testing models available

  1. In vitro stem cell model system for osteoblastogenesis
  2. In vitro stem cell model system for osteoclastogenesis
  3. In vitro stem cell model system for adipogenesis
  4. In vivo genetic toxicity testing using Micronucleus assay
  5. The diet-induced hyperlipidemic animal model system
  6. Acute oral toxicity testing in the rat model system
  7. In vivo anti-inflammatory study animal model
  8. In vitro biocompatibility and toxicity testing
  9. Wound healing assay in the rat model system
  10. In Vitro bacterial reverse mutation assay
  11. In vivo anti-arthritic study animal model
  12. In vitro cell toxicity using MTT assay
  13. In vitro cancer stem cell model
  14. In vitro cell clonogenic assay
  15. In vivo estrus cycle assessment in female Wistar rat  
  16. In vivo PCOS induction model
  17. In vivo Subacute oral toxicity study
  18. In vivo bioimaging of Candida albicans with a near-infrared dye
  19. In vitro model for peripheral blood mononuclear cells
  20. Green synthesis nanoparticle and testing
  21. In vivo granuloma study animal model

SOPs available: 103 list the names of sops

S.No. Facility No. of SOP
1 Microscopy and imaging 8
2 Microbiology and genetic toxicology facility 7
3 Clinical Chemistry facility 22
4 Cell Culture Facility 17
5 Small Animal Facility 11
6 Molecular Biology Facility 35
7 Pathology Facility 10
8 Genetic toxicology facility 1
  Total 111
S.No Facility SOP  No SOP Title
1 Microscopy and imaging CAReTS/MIM/E001 Operation and maintenance of LI- COR Odyssey Fc
2   CAReTS/MIM/E002 Operation and maintenance of LI- COR Pearl Trilogy
3   CAReTS/MIM/P001 Earthworm imaging
4   CAReTS/MIM/P002 Egg imaging
5   CAReTS/MIM/P003 Chick imaging
6   CAReTS/MIM/P004 Imaging of marine extracts
7   CAReTS/MIM/P005 Imaging of leaves
8   CAReTS/MIM/E003 Operation and Maintenance of EZ Anesthesia system 
9 Microbiology facility CAReTS/MGT/P001 Bacterial Aggregation assay
10   CAReTS/MGT/P002 In Vivo Mammalian micronucleus assay- Validation study
11   CAReTS/MGT/P003 Bacterial Biofilm assay
12   CAReTS/MGT/P004 MTT assay
13   CAReTS/MGT/P005 Demineralisation assay
14   CAReTS/MGT/P007 C.elegans medium preparation
15   CAReTS/MGT/P008 Microbial DNA isolation of fecal samples
16 Genetic Toxicology facility CAReTS/MGT/P006 AMES
17 Clinical Chemistry Facility CAReTS/CCH/E001 Operation and maintenance of Automated Clinical Biochemistry analyser Sysmex- BX 3010
18   CAReTS/CCH/E002 Operation and maintenance of Mindray BC-5000 Vet Auto Hematology Analyser
19   CAReTS/CCH/MBCP001 Sample collection and protocol for whole blood
20   CAReTS/CCH/E003 Operation and maintenance of Arkray Aution eleven AE-4020 Semi- Automatic Urine Analyzer
21   CAReTS/CCH/E004 Operation and maintenance of Cold storage system
22   CAReTS/CCH/BARP1 Preparation of Sysmex -3010X reagents for Automated Biochemical Clinical Analyzer
23   CAReTS/CCH/AA11P01 Sample collection for Urine and protocol for loading
24   CAReTS/CCH/P004 Evaluation of hemocompatibility of synthetic nanomaterial
25   CAReTS/CCH/M001 Biochemistry Analyser Sysmex-301 Maintenance
26   CAReTS/CCH/M002 Mindray BC-5000 Vet Hematology Analyser Maintenance
27   CAReTS/LAB/E001 Operation and maintaining of Hot air Oven
28   CAReTS/LAB/E002 Operation and maintenance of Eutech Cyber Scan 1100 pH meter
29   CAReTS/LAB/E003 Operation and maintenance of Autoclave
30   CAReTS/CCH/M003 Urine Analyzer Maintenance
31   CAReTS/LAB/ASE001 Cell culture material sterilization
32   CAReTS/LAB/ASE002 Microbiology medium and material sterilization
33   CAReTS/LAB/ASE003 Microbiology decontamination
34   CAReTS/LAB/E004 Operation and maintenance of Magnetic stirrer
35   CAReTS/LAB/E005 Operation and maintenance of vortex mixer
36   CAReTS/LAB/E006 Operation and maintenance of weighing balance
37   CAReTS/LAB/E007 Operation and maintenance of gas supply
38   CAReTS/LAB/ASE003 Microbiology Media Preparation
39 Cell Culture Facility CAReTS/CCF/E001 Operation and maintenance of CO2 Incubator 230AI
40   CAReTS/CCF/E002 Operation and maintenance of CO2 incubator 160I
41   CAReTS/CCF/E003 Operation and maintenance of Axiom Biosafety Cabinet
42   CAReTS/CCF/E004 Operation and maintenance of Safezone Biosafety cabinet
43   CAReTS/CCF/E005 Operation and maintenance of EVOS® FL Auto 2 Imaging
44   CAReTS/CCF/P001 Washing and Sterilization of Cell culturewares
45   CAReTS/CCF/P002 Preparation and sterilization of Cell culture Media
46   CAReTS/CCF/P003 Passaging /Splitting Adherent Cells
47   CAReTS/CCF/P004 Cyropreserving Animal Cells
48   CAReTS/CCF/P005 Reviving Cryopreserved Cells in Liquid nitrogen
49   CAReTS/CCF/P006 Cell counting Using Hemocytometer
50   CAReTS/CCF/P007 MTT Cell Proliferation assay
51   CAReTS/CCF/P008 Practice in cell culture
52   CAReTS/CCF/P008 Acridine Orange/Ethidium bromide staining assay
53   CAReTS/CCF/P010 Grafting of human tumor cells on chick embryo
54   CAReTS/CCF/P011 Preparation of 70% ethanol
55   CAReTS/CCF/P012 Preparation of chromic acid for glass wash
56 Small Animal Facility CAReTS/SAF/F001 Operation of Individually Ventilated cage
57   CAReTS/SAF/F002 Operation and maintenance of Small animal cage change station
58   CAReTS/SAF/P001 Maintenance of animal and housing
59   CAReTS/SAF/P002 Feeding
60   CAReTS/SAF/P003 Necropsy
61   CAReTS/SAF/P004 Decontamination
62   CAReTS/SAF/F008 Restraining of Laboratory Animals
63   CAReTS/SAF/F009 Determination of sex
64   CAReTS/SAF/F010 Operation of Euthanasia chamber
65   CAReTS/SAF/P001 Application of Terminalia arjuna (Roxb.) wight & Arn Leaf extract in Wound Healing
66   CAReTS/SAF/P002 Estrus Cycle
67 Molecular Biology Facility CAReTS/MBY/E001 Operation and maintenance of Multiscan GO Microplate Spectrophotometer
68   CAReTS/MBY/E002 Operation and maintenance of Qiagen QIA cube
69   CAReTS/MBY/P001 Sodium Dodecyl Sulfate Polyacrylamide Gel Electrophoresis (SDS-PAGE) –Manual method
70   CAReTS/MBY/P002 DPPH- Free Radical scavenging activity
71   CAReTS/MBY/P003 DNA/RNA quantification
72   CAReTS/MBY/P004 ELISA assay
73   CAReTS/MBY/P005 Estimation of Catalase 
74   CAReTS/MBY/P006 Estimation of SOD
75   CAReTS/MBY/P007 Estimation of GSH 
76   CAReTS/MBY/P008 Estimation of TBARS
77   CAReTS/MBY/P009 Estimation of Catalase 
78   CAReTS/MBY/P010 Isolation of DNA from fecal sample
79  CAReTS/MBY/P011 Isolation of RNA from tissue sample using Rnase mini kit  
80   CAReTS/MBY/P012 Isolation of RNA from tissue sample using phenol chloroform 
81   CAReTS/MBY/P013 Extraction and Purification of DNA from Buccal swab 
82   CAReTS/MBY/P014 Isolation of DNA from Bacterial 
83   CAReTS/MBY/P015 Extraction and Purification of DNA from Buccal epithelial cells 
84   CAReTS/MBY/P016Transfer of Blot-Semi Dry
85   CAReTS/MBY/P017 Transfer of Blot-wet
86   CAReTS/MBY/P018 Westernblotting
87   CAReTS/MBY/P019 Gel and membrane quantification
88   CAReTS/MBY/E003 Operation and maintenance of mini transblot
89   CAReTS/MBY/E004 Operation and Maintenance of vertical Gel caster
90   CAReTS/MBY/E005 Operation and Maintenance of vertical gel electrophoresis
91   CAReTS/MBY/E006 Operation and Maintenance of rocker
92   CAReTS/MBY/E007 Operation and Maintenance of mini centrifuge
93   CAReTS/MBY/P019 GEL Staining–coomassie brilliant blue and destaing method
94   CAReTS/MBY/E008 Operation and Maintenance of Rotor Q Gene
95   CAReTS/MBY/E001 Operation and maintenance of Multiscan GO Microplate Spectrophotometer
96   CAReTS/MBY/E002 Operation and maintenance of Qiagen QIA cube
97   CAReTS/MBY/P001 Sodium Dodecyl Sulfate Polyacrylamide Gel Electrophoresis (SDS-PAGE) –Manual method
98   CAReTS/MBY/P002 DPPH- Free Radical scavenging activity
99   CAReTS/MBY/P003 DNA/RNA quantification
100   CAReTS/MBY/P004 ELISA assay
101 Pathology Facility CAReTS/PAT/E001 Operation and maintenance of Automated Tissue Processor
102   CAReTS/PAT/E002 Operation and maintenance of Tissue Embedding System
103   CAReTS/PAT/E003 Operation and maintenance of Manual Rotary Microtome
104   CAReTS/PAT/P001 Hematoxylin And Eosin Staining
105   CAReTS/PAT/P002 Decalcification of Bone
106   CAReTS/PAT/P003 Periodic acid-Schiff’s (PAS) staining
107   CAReTS/PAT/P004 Masson’s Trichrome Staining
108   CAReTS/PAT/P005 Giemsa’s Stain for Micronuclei identification from bone marrow
109   CAReTS/PAT/E004 Operation and Maintenance of Tissue Flotation Bath
  • +91 - 0413 – 2615449 to 58
  • [email protected]
  • SRI BALAJI VIDYAPEETH
    SBV Campus
    Pillaiyarkuppam
    Pondicherry - 607 402.
    India.

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